Features of RSpak DE Series (2) (Adsorption of Metal Coordination Compound)
Since hinokititol and oxin-Cu are strong metal coordination compounds, they are adsorbed with the impurity metals in
silica gel and the adsorption causes peak tailing. In case of RSpak
DE series (polymer-based columns for
reversed phase chromatography), the column is free from the effect of metals and sample will not be adsorbed by the
column.
Sample : 5micro-L
(Left)
1. 0.01% Hinokitiol
2. 0.001% Benzene
(Right)
1. 0.01% Oxine-Cu
Column : (Left); Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : (Left); (1mM EDTA + 20mM KH2PO4) aq./CH3CN=60/40
(Right); 20mM H3PO4 aq./CH3CN=95/5
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : 40°C