Features of RSpak DE Series (2) (Adsorption of Metal Coordination Compound)

Since hinokititol and oxin-Cu are strong metal coordination compounds, they are adsorbed with the impurity metals in
silica gel and the adsorption causes peak tailing. In case of RSpak
DE series (polymer-based columns for
reversed phase chromatography), the column is free from the effect of metals and sample will not be adsorbed by the
column.

Sample : 5micro-L
(Left)

1. 0.01% Hinokitiol

2. 0.001% Benzene

(Right)

1. 0.01% Oxine-Cu

 Column : (Left); Shodex RSpak DE-413 (4.6mmID*150mm) 

 Eluent : (Left); (1mM EDTA + 20mM KH2PO4) aq./CH3CN=60/40
 (Right); 20mM H3PO4 aq./CH3CN=95/5

 Flow rate : 1.0mL/min

 Detector : UV(254nm)

 Column temp. : 40°C