To realize the best performance of RSpak DE-413 column, we recommend to use the following HPLC system with the column.
| Device | Recommended specifications and/or setting | Example |
|---|---|---|
| Pump | Flow rate: max. 1mL/min. | |
| Injector | Low dead volume type
Sample loop: max. 50micro-microL(5micro-L loop is recommended) |
|
| From injector
to column |
0.1 to 0.13mmID, 100mm length piping | |
| From column
to detector |
0.1mmID piping | |
| Detector | Detector with a micro cell should be used.
Response should be set to “FAST”. |
Shodex RI-104
(small cell volume) |
Sample : (A); 1.5micro-L, (B); 5micro-L
1. Methylethyl ketone 0.2%
2. Diethyl ketone 0.2%
3. Di-n-propyl ketone 0.4%
4. Di-n-butyl ketone 0.6%
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : CH3CN/H2O=50/50
Flow rate : 1.0mL/min
Detector : UV(262nm)
Column temp. : 40°C
