Di-n-butyl ketone were analyzed using RSpak
(a column for reversed phase chromatography). When the sample concentration was constant,
a decrease of column efficiency was observed accroding to an icrease of injection volume.
The decrease started from 50micro-L and it was marked when the injection volume exceeded 100micro-L.
To the contrary, when the injection volume was constant, a decrease of column efficiency was not
observed until sample load exceeded 500micro-g.
Sample : Di-n-butyl ketone
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : CH3CN/H2O=50/50
Flow rate : 1.0mL/min
Detector : UV(262nm)
Column temp. : 40°C