Fibrinogen was analyzed using AFpak ALC-894 (an affinity column).
Sample : Fibrinogen (Sigma)
Column : Shodex AFpak ALC-894 (8.0mmID*50mm)
Eluent : (A); 0.05M Tris-HCl(pH7.4) + 0.15M NaCl + 0.5mM CaCl2 + 0.5mM MnCl2
(B); (A) + 0.1M Methyl mannoside
Step gradient: (A) to (B)
Flow rate : 0.5mL/min
Detector : UV(280nm)
Column temp. : Room temp.
