Vitamins in food were analyzed using RSpak DE-413, a column for reversed phase chromatography.
(Sample pretreatment)
– Dissolve 80mg of the food containing vitamins in 12mL of the eluant (A).
– Pass through a 0.45μm filter before injecting into HPLC.
| Concentration | Contents in 4g | |
|---|---|---|
| 1. Vitamin C |
145.8micro-g/mL
|
87.5mg
|
| 2. Vitamin B6 |
21.2micro-g/mL
|
12.7mg
|
| 3. Vitamin B2 |
7.3micr-og/mL
|
4.4mg
|
Column : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent : (A); 1/30M Potasium phosphate buffer(pH6.8)/CH3CN=98/2
(B); H2O/CH3CN=50/50
gradient: 0min to 4min, 100% (A),
4min to 15 min, 100% (A) to 30% (A),
After 15 min, 30% (A)
Flow rate : 0.6mL/min
Detector : UV(254nm)
Column temp. : 35℃
