The relative hydrophilicity of gluconic acids with similar molecular structures can be decreased due to their suppressed dissociation in the acidic eluent. Thus, they are retained in RSpak DE-613 column by reversed phase mode and separated.
Sample :
1. HCl
2. 2-Ketogluconic acid
3. Gluconic acid
4. 5-Ketogluconic acid
5. Tartaric acid
6. Glycolic acid
Column : Shodex RSpak DE-613 (6.0mmI.D. x 150mm) Eluent : 2mM HClO4 aq. Flow rate : (Eluent); 0.5mL/min, (Reagent); 0.2mL/min Detector : VIS(430nm) (Post column method) Column temp. : 30 to 32°C
