AFpak AGT-894 (an affinity column) has glutathione (sulfohydrate present in large quantity in living organisms) as a ligand.
Sample : Glutathione S-transferase
Column : Shodex AFpak AGT-894 (8.0mmID*50mm)
Eluent : (A); 22mM Phosphate buffer(pH7.0)
(B); 50mM Tris buffer(pH9.6) + 5mM Glutathione
Step gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.
