Avidin, one of the saccharide-binding proteins, was analyzed using aqueous solvent SEC (GFC) column, PROTEIN KW-804.
Sample: 50μL
1. Avidin (Wako Pure Chemical Industries, Ltd.) 0.1%.
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Column : Shodex PROTEIN KW-804 (8.0mmI.D. x 300mm) Eluent : 100mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl Flow rate : 1.0mL/min Detector : UV(254nm) Column temp. : 30°C