Glycoprotein Avidin

Avidin, one of the saccharide-binding proteins, was analyzed using aqueous solvent SEC (GFC) column, PROTEIN KW-804.

Sample: 50μL
1. Avidin (Wako Pure Chemical Industries, Ltd.) 0.1%.

Column       : Shodex PROTEIN KW-804 (8.0mmI.D. x 300mm)
Eluent       : 100mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 30°C