Glyphosate and Glufosinate

Glyphosate and glufosinate which are phosphorus-containing amino acid type herbicides were analyzed with IC/ICP-MS using IC SI-90 4E (a column for anion chromatography).

Sample : 20micro-L
1. Glufosinate
2. Glyphosate

 Column : Shodex IC SI-90 4E (4.0mmID*250mm)
 Eluent : (A) 0.3mM NaOH aq.
 (B) 0.3mM NaOH + 20mM Na2CO3 aq.
 Gradient:
 0 to 20min, A/B=95/5 
 20 to 30min, A/B=95/5 to 50/50
 30 to 40min, A/B=50/50
 Flow rate : 1.0mL/min
 Suppresor : Dionex AMMS-ICS II
 Detector : ICP/MS, P at m/z=31
 Column temp. : Room temp.