In the past, vitamins were typically analyzed by bioassay or enzyme assay. However, these methods were complicated and differed according to the kind of vitamins to be analyzed, it was impossible to analyze several kinds of vitamins at one time. Recently, HPLC methods have been adopted as one of the official methods in Japan. Specifically, HPLC analysis using reversed phase mode or normal phase mode is widely accepted for vitamin analysis.
(Hormones and Neurotransmitters)
Hormones of mammalia can be classified into peptides, amino acids, steroids, catecholamines, etc. Most columns used in the section of “Proteins, Peptides and Amino Acids” also can be used for the analysis of hormones. Reversed phase mode is especially suitable for the analysis of steroids and catecholamines.
Reversed phase, normal phase, and GFC modes are used for the analysis of lipids.