HEETs (Hydroxyeicosatetraenoic Acids)

HETEs (hydroxyeicosatetraenoic acids) were analyzed using Asahipak ODP-50 6D ( a column for reversed phase chromatography ).

Sample : HETE
1. 15-HETE
2. 12-HETE
3. 5-HETE

 Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
 Eluent : H2O/CH3COOH/CH3CN/CH3OH=50/0.1/50/10
 Flow rate : 1.0mL/min
 Detector : UV(235nm)
 Column temp. : 30°C
 

Courtesy of Dr. Oishi and Dr. Yamaki, Kitazato University