Hippuric Acid etc. in Urine (2)

Some metabolites in urine have been added to the health check as special items in Japan recently. From the items, hippuric acid, methylhippuric acid(o-, m-, p-), mandelic acid which are metabolites of toluene, xylene and styrene and creatinine were analyzed using RSpak DE-413 (a column for reversed phase chromatography) within 10 minutes.

Sample :
(Left); Standards
1. Creatinine
2. Mandelic acid
3. Hippuric acid
4. o-Methylhippuric acid
5. p-Methylhippuric acid
6. m-Methylhippuric acid

(Right); Urine 100-fold diluted

 Column : Shodex RSpak DE-413 (4.6mmID*150mm)
 Eluent : CH3CN/Buffer=1/5
 (Buffer: 20mM Sodium phophate + 20mM beta-Cyclodextrin + 4.5mM sodium dodecyl sulfate(pH3.2))
 Flow rate : 1.0mL/min
 Detector : UV(225nm)
 Colomn temp. : 50°C