Histidine and 1-Methylhistidine

Histidine and 1-methylhistidine were analyzed using Asahipak ODP-50 6D (a column for reversed-phase chromatography).

Sample: 0.005% each, 5μL

1. Histidine

2. 1-Methylhistidine

Column       : Shodex Asahipak ODP-50 6D (6.0mmI.D. x 150mm)
Eluent       : 10mM Na2HPO4 aq.
Flow rate    : 0.5mL/min
Detector     : UV(210nm)
Column temp. : 30°C