Histidine and 1-methylhistidine were analyzed using Asahipak ODP-50 6D (a column for reversed-phase chromatography).
Sample: 0.005% each, 5μL
1. Histidine
2. 1-Methylhistidine
Column : Shodex Asahipak ODP-50 6D (6.0mmI.D. x 150mm) Eluent : 10mM Na2HPO4 aq. Flow rate : 0.5mL/min Detector : UV(210nm) Column temp. : 30°C