Human gamma-globulin was analyzed using AFpak APR-894 (an affinity column).
Sample :
Globulin
Human gamma-globulin (Sigma)
Column : Shodex AFpak APR-894 (8.0mmID*50mm)
Eluent : (A); 0.01M Sodium phosphate buffer(pH6.5)
(B); (A) + 1.0M NaCl
Linear gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.
