The ratio of human mercaptoalbumin(HMA) to human nonmercaptoalbumin(HNA) in serum is thought to have some relation with aging. The correlation between the ratio and various types of illness is being studied. Ion exchange mode has been used most commonly for the analysis of albumin, however, as isocratic elution widens the peaks, gradient elution must be applied in ion exchange mode. In contrast, one of the characteristics of Asahipak GS-520 ( a multimode column ) is its capacity of enabling isocratic elution of HMA and HNA with isocratic elution using simple eluent such as phosphate buffer. This is the original Asahipak GS-520 column separation method which allows proteins other than albumin to elute first in the GFC mode and only albumin to elute later.
GS-520 7E is an old type of GS-520 HQ.
fHMA = (HMA)/(HMA + HNA)
Columns : Shodex Asahipak GS-520 7E (7.6mmID*250mm) x 8
Eluent : 0.03M Sodium phosphate buffer(pH6.87) + 0.15M Na2SO4
Flow rate : 0.8mL/min
Detector : UV(280nm)
Column temp. : 28°C
Coutesy of Dr. Sogami, Fujita Health University, School of health Science