Human serum was analyzed using IEC DEAE-825 ( a column for waek anion exchange chromatography ).
Sample : Human serum
Column : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent : (A); 50mM Tris-HCl buffer(pH8.0)
(B); (A) + 0.5M NaCl
Linear gradient: 0min to 60min, (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.
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