Human serum albumin was analyzed using AFpak APD-894 (an affinity column).
Sample : Human serum albumin (Sigma)
Column : Shodex AFpak APD-894 (8.0mmID*50mm)
Eluent : (A); 0.02M Tris-HCl buffer(pH5.0)
(B); (A) + 2.0M KCl
Step gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.
