HVA and VMA in Urine

Homovanilic acid (HVA) and Vanillylmandelic acid (VMA) in urine are analyzed using Asahipak GS-320 7E ( a multimode column ) and electrochemical detection (ECD).
GS-320 7E is an old type of GS-320 HQ.

Sample : HVA and VMA

 Column : Shodex Asahipak GS-320 7E (7.6mmID*250mm)
 Eluent : 0.5M Citric buffer + 0.1M KCl (pH7.0)
 Flow rate : 1.0mL/min
 Detector : ECD(800mV vs Ag/AgCl)
 Column temp. : 25°C

Courtesy of Dr. Iriyama, The Jikei University, School of Medicine