Three kinds of hyaluronic acid with MW of one million or more was analyzed with OHpak SB-807 HQ. Since estimated exclusion limit of SB-807 HQ is 5.0 x 108g/mol, Hyaluronic acids elute with size exclusion mode.
Sample: Hyaluronic acid, 100μL
Column : Shodex OHpak SB-807 HQ (8.0mmI.D. x 300mm) Eluent : 0.2M NaCl aq. Flow rate : 0.5mL/min Detector : Shodex RI Column temp. : 30°C
