Hydantoins

Five kinds of hidantoins were analyzed using RSpak DE-613 with aqueous eluent. The elution order of the hidantoins is the order of hydrophilicity of R-group.

Sample : Hydantoins, 0.05% each, 3micro-L
1. 5-Carboxyethyl Hydantoin
2. 5-Hydroxymethyl Hydantoin
3. Hydantoin
4. 5-Methyl Hydantoin
5. 5-Ethyl Hydantoin

 Column : Shodex RSpak DE-613 (6.0mmID*150mm)
 Eluent : 1/30M Na2HPO4 + 1/30M KH2PO4 aq.
 Flow rate : 2.0mL/min
 Detector : UV(210nm)
 Column temp. : 50°C