Hydrolyzed Dextran (1)

Asahipak GS-220 HQ is the best suitable column for the analysis of oligosaccharides up to 10mers performing under GFC mode. In many cases, compounds can be eluted with isocratic elution of aqueous eluent.
(A): Rapid analysis was performed using one column of GS-220 HQ.
(B): Flow rate was reduced using one column of GS-220 HQ.
(C): Two columns of GS-220 HQ were used to realize better separation.

Sample : Dextran, Hydrolyzed dextran
1. Glucose
2. Isomaltose
3. Isomaltotriose
4. Isomaltotetraose
5. Isomaltopentaose
6. Isomaltohexaose
7. Isomaltoheptaose
8. Isomaltooctaose
9. Isomaltononaose
10. Isomaltodecaose

 Columns : (A), (B); Shodex Asahipak GS-220 HQ x 1, (C); GS-220 HQ x 2 (7.6mmID*300mm each)
 Eluent : H2O
 Flow rate : (A); 0.5mL/min, (B); 0.2mL/min, (C); 0.3mL/min
 Detector : Shodex RI
 Column temp. : 60°C