(Sample preparation)
1) Hydrolyze 4% dextran aquaous solution using 1M HCl at 100 °C.
2) Take sample before and after 30, 40 and 50min from the start of the hydrolyzation.
3) Neutralize the sample adding cold 1M NaOH aaquaous solution.
4) Desalt the sample passing it through ion-exchange resin.
5) Filtrate with 0.45 micron membrane filter.
Sample : Hydrolyzed dextran, 20micro-L (Dextran)
Columns : Shodex SUGAR KS-802(8.0mmID*300mm) x 2 Eluent : H2O Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 80°C
