Hydrophile-Lipophile Balance (HLB) was analyzed using Asahipak GS-310 7G ( a multisolvent GPC column ). HLB is one of the indicators representing the ratio of the hydrophilicity of a surfactant to its hydrophobicity. The HLB value and elution volume of octylphenol ethoxylate with a different polymerization degree show a good correlation. Therefore, it is possible to measure the HLB value from the elution volume.
GS-310 7G is an old type of GF-310 HQ.
Sample : Octylphenol ethoxylate
Column : Shodex Asahipak GS-310 7G (7.6mmID*500mm)
Eluent : H2O/CH3CN=50/50
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : 30°C