Hydroxyethylcellulose, a nonionic polysaccharide, is used as a binder, thickening agent, etc. When analyzing a high-polarity polymer, the chromatogram may be abnormal due to intermolecular hydrogen bonding. The addition of salt to the eluent will decrease the hydrogen bonding interactions. The effect of increased salt concentration in the eluent is shown for the analysis of f hydroxyethyl cellulose. When water is used as the eluent, hydroxyethylcellulose is eluted fast and the chromatogram shows three peaks. The chromatogram is improved with the addition of salt. The peak shape of hydroxyethyl cellulose stabilizes at a low salt concentration.
Please refer to “Carboxymethylcellulose (3) (SB-806M HQ)”
Sample : 50μL
Hydroxyethyl cellulose (800-1,500 cP) 0.1%
Columns : Shodex OHpak SB-806M HQ (8.0mmI.D. x 300mm) x 2 Eluent : H2O or NaCl aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40°C