Hydroxypropyl-β-cyclodextrin is added to pharmaceuticals, including vaccines, to improve the solubility in water, stabilizing, etc. It is also expected to be used as *adjuvant in recent years. In this application, trypsin inhibitor and hydroxypropyl-β-cyclodextrin were separated using OHpak SB-802.5 HQ. It is possible to measure and quantify hydroxypropyl-β-cyclodextrin without deproteinization by using a column for SEC. The calibration curve shows good linearity in the range from 0.05 to 10mg/mL.
*Adjuvant: Generic term for factors that enhance the effect of vaccines
Sample : 1mg/mL each, 50µL
1. Trypsin Inhibitor
(MW: 20,000)
2. Hydroxypropyl-β-cyclodextrin
Columns : Shodex OHpak SB-802.5 HQ (8.0mmI.D. x 300mm) x 2 Eluent : **PBS(-)/CH3CN=80/20 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 30°C **PBS(-) Phosphate buffered saline without calcium and magnesium
