The relations between the theoretical plate number (TPN) and flow rate and
also between retention time and flow rate were investigated using ODP2
HP-4D column (4.6mm ID x 150mm length). Each sample showed the highest
TPN at the flow rate of 0.4mL/min, while efficiency decreased at 0.3mL/min
and below due to sample diffusion. Also, each sample showed extremely long
retention times at 0.5mL/min and below. Therefore a flow rate of 0.5 –
1.0mL/min is recommended for general analysis using ODP2 HP-4D. Flow rate
of 0.1 – 0.2mL/min are recommended for ODP2 HP-2D (2mm ID x 150mm length).
* Ratio of TPN [%] : TPN at a flow rate of 0.5mL/min is
set as 100%.
Column : Shodex ODP2 HP-4D (4.6mmID*150mm)
Eluent : H2O/CH3CN=55/45
Detector : UV(254nm)
Column temp. : 40°C