The influence of injection volume on theoretical plate numbers (TPN) is investigated for organic SEC semi-micro columns GPC KF-402HQ and KF-406LHQ. Injection volume of 5µL or less is appropriate for KF-402HQ and injection volume of 20µL or below is adequate for using 2 KF-406LHQ columns.
Sample : Dicyclohexylphthalate
Columns : Shodex GPC KF-402HQ, KF-406LHQ x 2 (4.6mmID*250mm each)
Eluent : THF
Flow rate : 0.3mL/min
Column temp. : 25°C