We can say the same thing regarding RI detector. The cell volume of RI detector affects the plate number of the peaks. The plate number when RI-101 detector is used is a half of that when RI-104 designed for down sized chromatography is used. (Fig.2)
* When the detector for down sized chromatography is used, it is needed to prepeare the instruments including the columns and the injector designed for the down sized chromatography to get good performance.
Sample :
1. PS, Polystyrene (MW: 66,000)
2. PS, Polystyrene (MW: 3,250)
3. PS, Polystyrene (MW: 580)
4. Propylbenzene
5. Benzene
(Fig.1) (Fig.2)
Columns : Shodex GPC KF-402HQ Shodex GPC KF-402HQ (4.6mmID*250mm) x 2
Eluent : THF THF
Flow rate : 0.3mL/min 0.3mL/min
Column temp. : 40°C 25°C