LC/MS is effective for the high sensitivity analysis of drugs; however, when
protein is present and enters the MS (Mass Detector), it contaminates the
MS or suppresses ionization of the sample. Pretreatment does not often remove
protein thoroughly. Drugs in biological fluid are hard to analyze because
protein co-elutes with the component of interest. The target drug receives
ion suppression from the protein and appears as a small peak.
ODP2 HP can separate the target
from protein by eluting protein early and clearly. Barbital was introduced
into the MS by a switching valve after BSA (protein) was eluted, and barbital
was detected without any influence of ion suppression.
Sample : Barbital (500ng/mL) in BSA (7.0mg/L),
10µL
Column : Shodex ODP2 HP-2B (2.0mmID*50mm esch)
Eluent : 10mM Ammonium acetate aq./CH3CN=70/30
Flow rate : 0.2mL/min
Detector : UV(280nm), ESI-MS(SIM negative)
Column temp. : 30°C
