The relation between sample injection volume and theoretical plate number (TPN) for ODP2 HP-4D (4.6mm ID x 150mm length) is shown. Sample injection volume of 40µL and below is recommended for ODP2 HP-4D to achieve maximum efficiency. Sample injection volume of 8µL and below is recommended for ODP2 HP-2D (2mm ID x 150mm length).
Sample : 0.6µL each
Phenol
Methyl benzoate
Caffeine
Ratio of TPN [%] : TPN at sample injection of 2µL is set as 100%.
Column : Shodex ODP2 HP-4D (4.6mmID*150mm)
Eluent : H2O/CH3CN=55/45
Flow rate : 1.0mL/min
Detector : UV(254nm) Phenol, Methyl benzoate
UV(300nm) Caffeine
Column temp. : 40°C
