The relation between sample loads and thepretical plate number (TPN) for ODP2 HP-4D(4.6mm ID x 150mm length) is shown. Sample loads of 10µg or less are recommended with ODP2 HP-4D for best column performance and sample loads of 2µg or below are recommended for ODP2 HP-2D (2mm ID x 150mm length).
Sample : 5µL
Methyl benzoate 350ppm
Ratio of TPN [%] : TPN at sample load of 5µg is set as 100%.
Column : Shodex ODP2 HP-4D (4.6mmID*150mm)
Eluent : H2O/CH3CN=55/45
Flow rate : 1.0mL/min
Detector : UV(254nm) Phenol, Methyl benzoate
Column temp. : 40°C