The relation between column temperature and retention time and the relation between column temperature and theoretical plate number (TPN) for ODP2 HP 4D (4.6mm ID x 150mm length) are shown. As retention time and TPN vary with changes in column temperature, the use of a column oven for temperature control is recommended.
Sample : 5µ-L
Methyl benzoate 350µ-g/mL
Ratio of TPN [%] : TPN at 40°-C is set as 100%.
Column : Shodex ODP2 HP-4D (4.6mmID*150mm)
Eluent : H2O/CH3CN=55/45
Detector : UV(254nm)
Column temp. : 40°C