Lactate Dehydrogenase

L-Lactate dehydrogenase was analyzed using AFpak ANA-894 (an affinity column).

Sample : L–Lactate dehydrogenase

 Column : Shodex AFpak ANA-894 (8.0mmID*50mm)
 Eluent : (A); 0.01M Potassium phosphate buffer(pH7.0)
 (B); (A) + 0.5M KCl
 Step gradient: (A) to (B)
 Flow rate : 1.0mL/min
 Detector : UV(280nm)
 Column temp. : Room temp.