Creatine, the precursor of creatine (guanidinoacetic acid), and the final metabolite of creatine (creatinine) were separated using a polymer-based amino column, Asahipak NH2P-40 2D, under HILIC mode. Hydrophilic compounds were retained at a high concentration of acetonitrile under HILIC mode condition. This is advantageous for the high sensitivity analysis by ESI-MS/MS.
Sample : 5μL
3. Guanidinoacetic acid
Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm) Eluent : 20mM HCOONH4 aq./CH3CN=30/70 Flow rate : 0.2mL/min Detector : ESI-MS/MS(MRM Positive) Column temp. : 30°C