LC/MS analysis of drugs in BSA was performed using polymer-based column ODP2
HP. Each drug has peak areas between 83 -100 % (peak areas without BSA are
set as 100 %), which means a good separation without receiving ion suppression
from protein.
Sample : drugs 500ng/mL each in BSA (7.0mg/L), 5µL
Lidocaine, Chlorpheniramine, Propranolol, Diphenhydramine, Bupivacaine, Verapamil, Imipramine
Column : Shodex ODP2 HP-2B (2.0mmID*50mm) Eluent : 10mM Ammonium acetate aq./CH3CN=70/30 Flow rate : 0.2mL/min Detector : UV(280nm), ESI-MS (SIM positive) Column temp. : 40°C