This shows LC/MS analysis of drugs in BSA with a gradient using polymer-based
ODP2 HP. Each drug has peak areas
between 98 – 107 % (peak areas without BSA are set as 100 %), which
means a good separation without receiving ion suppression from protein.
Sample : drugs 500ng/mL each in BSA (7.0mg/L), 5µL
Column : Shodex ODP2 HP-2B (2.0mmID*50mm)
Eluent : (A);10mM Ammonium acetate aq.
(B);100mM Ammonium acetate aq./CH3CN=10/90
gradient; (A) to (A) to 70%(B) to 70%(B) to (A),
0min to 5min to 10min to 15min to 15.01min
Flow rate : 0.2mL/min
Detector : ESI-MS (SIM positive)
Column temp. : 40°C