LC/MS analysis of drugs in control serum after removing proteins was done
by polymer-based column ODP2 2B HP.
Each drug has peak areas between 82 – 111 % (peak areas without BSA
are set as 100 %), which means a good separation without receiving ion suppression
from protein.
Sample: drugs 500ng/mL each in Control serum, 5µL
Lidocaine,
Chlorpheniramine,
Propranolol,
Diphenhydramine,
Bupivacaine,
Verapamil,
Imipramine
Column : Shodex ODP2 HP-2B (2.0mmID*50mm) Eluent : 10mM Ammonium acetate aq./CH3CN=70/30 Flow rate : 0.2mL/min Detector : UV(280nm), ESI-MS (SIM positive) Column temp. : 40°C
