LC/MS Analysis of Haloacetic Acids JJ-50 2D

Haloacetic acid is one type of disinfection byproducts (DBPs) generated
during water purification. The lower limit set by drinking-water quality
control law in Japan are; trichloroacetic acid (TCAA). In the past, GC/MS was used as the standard
method for haloacetic acid analysis, but recently LC/MS method has also
become a standard method (April, 2012 in Japan). Three haloacetic acids
were simultaneously analyzed by a multi-mode column, RSpak JJ-50 2D, using isocratic condition.

Sample : Haloacetic acid

(Standard) 2ng/mL each, 50micr-L
MCAA, Chloroacetic acid
DCAA, Dichloroacetic acid
TCAA, Trichloroacetic acid

Tap water
(Water), 50?L

Column : Shodex RSpak JJ-50 2D (2.0mmI.D. x 150mm)
 Eluent : 25mM CH3COONH4 aq.(pH9.2)/CH3CN=50/50
 Flow rate : 0.2mL/min
 Detector : ESI-MS
 MCAA?SIM 93(-)
 DCAA?MRM 127?83(-)
 TCAA?SIM 161(-)
 Column temp.?40°C