LC/MS Analysis of Low Molecular Weight Peptides (VC-50 2D)

Physiological functions of low molecular weight peptides (dipeptides, tripeptides) have been drawing attention in recent years. In this application, dipeptides and tripeptides were analyzed using HILICpak VC-50 2D, a polymer-based HILIC column, with LC/MS detection. The peptides containing basic amino acids tend to be  retained longer.

Sample : 5μL
50ng/mL each (in H₂O)
Ser-Glu
Gly-Tyr
Gly-Gly-Leu
Thr-Gln
Gly-Gly-Ala
Gly-Gln
Arg-Asp
Gly-Gly-His

Column       : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm)
Eluent       : (A); 100mM HCOOH aq. (B); CH3CN
               Linear gradient (High pressure);
               (B%) 60% (0 to 5min)
                    60% to 10% (5 to 6min)
                    10% (6 to 15min)
                    60% (15.1 to 20min)
Flow rate    : 0.3mL/min
Detector     : ESI-MS (SIM Positive)
Column temp. : 40°C