Physiological functions of low molecular weight peptides (dipeptides, tripeptides) have been drawing attention in recent years. In this application, dipeptides and tripeptides were analyzed using HILICpak VC-50 2D, a polymer-based HILIC column, with LC/MS detection. The peptides containing basic amino acids tend to be retained longer.
Sample: 5μL 50ng/mL each (in H2O)
Column : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm) Eluent : (A); 100mM HCOOH aq. (B); CH3CN Linear gradient (High pressure); (B%) 60% (0 to 5min) 60% to 10% (5 to 6min) 10% (6 to 15min) 60% (15.1 to 20min) Flow rate : 0.3mL/min Detector : ESI-MS (SIM Positive) Column temp. : 40°C