Cyanuric acid is a structural analogue of melamine, and thus sometimes detected as an impurity of melamine. In this application, polymer-based amino column, NH2P40-2D, was used to separate melamine and cyanuric acid using HILIC mode. Hydrophilic compounds are retained at a high concentration acetonitrile under HILIC mode condition. This is advantageous for the high sensitivity analysis by ESI-MS.
Column? : Shodex NH2P40-2D (2.0mmID*150mm)
Eluent : 50mM Ammonium formate aq./CH3CN=25/75
Flow rate : 0.2mL/min
Detector : Melamine; ESI-MS/MS
Cyanuric acid; ESI-MS
Column temp. : 30°C
