LC/MS Analysis of N-Acetyl-chitooligosaccharides

Sugar analysis column, NH2P40-2D, was used with a LC/MS to separate N-Acetyl-chitooligosaccharides. By adding ammonia to the solvent, it enhances the ionization of saccharides, and thus provides high sensitivity detection.

Sample : 5µL, Mixture of
N-Acetyl-chitooligosaccharides

(Seikagaku Co. code:800104 Chitooligosaccharides Mixture)
500ng/mL

1. N-Acetyl-D-glucosamine

2. di-N-Acetyl-chitobiose

3. tri-N-Acetyl-chitotriose

4. tetra-N-Acetyl-chitotetraose

5. penta-N-Acetyl-chitopentaose

6. hexa-N-Acetyl-chitohexaose

Column : Shodex NH240-2D (2.0mmID*150mm)
 Eluent : (A); 0.05% Ammonia aq.?(B); CH3CN
 Linear gradient; 
 (B%) 75% (0min) ? 50% (10min) ? 50% (15min)
 Flow rate : 0.2mL/min
 Detector : ESI-MS(SIM Negative)
 Column temp. : 30°C