Sugar analysis column, NH2P40-2D, was used with a LC/MS to separate N-Acetyl-chitooligosaccharides. By adding ammonia to the solvent, it enhances the ionization of saccharides, and thus provides high sensitivity detection.

Sample : 5µL, Mixture of
N-Acetyl-chitooligosaccharides
(Seikagaku Co. code:800104 Chitooligosaccharides Mixture)
500ng/mL
4. tetra-N-Acetyl-chitotetraose
Column : Shodex NH240-2D (2.0mmID*150mm)
Eluent : (A); 0.05% Ammonia aq.?(B); CH3CN
Linear gradient;
(B%) 75% (0min) ? 50% (10min) ? 50% (15min)
Flow rate : 0.2mL/min
Detector : ESI-MS(SIM Negative)
Column temp. : 30°C