A polymer-based reversed phase column, RSpak DE-213, was used with a LC/MS to separate organic acids.
Sample : 50ng/mL each, 10μL
Glyoxylic acid
Pyruvic acid
Glycolic acid
Lactic acid
Maleic acid
Succinic acid
Fumaric acid
Adipic acid
Column : Shodex RSpak DE-213 (2.0mmI.D. x 150mm)
Eluent : (A); 0.1%(v/v) Formic acid aq.
(B); CH3CN
Linear gradient:
(B%) 5% (0min) -> 5% (2min) -> 15% (2.5min) -> 15% (10min)
Flow rate : 0.2mL/min
Detector : ESI-MS (SIM Negative)
Column temp. : 30°C
