LC/MS Aanalysis of Pantothenic Acid and Vitamin C (VT-50 2D)

Pantothenic acid and ascorbic acid were analyzed by HILICpak VT-50 2D under the conditions of HILIC mode LC/MS.

Sample : 100ng/mL, 10μL
1. Pantothenic acid (Vitamin B₅)
2. Ascorbic acid (Vitamin C)

Column       : Shodex HILICpak VT-50 2D (2.0mmI.D. x 150mm)
Eluent       : 50mM HCOONH4 aq./CH3CN=30/70
Flow rate    : 0.2mL/min
Detector     : ESI-MS (SIM)
Column temp. : 30°C