Histamine is a neurotransmitter and it also is well known as a causative agent of food poisoning. In this application, HILICpak VC-50 2D was used for a LC/MS/MS analysis of histidine which is precursor of histamine. The two components can be simultaneously analyzed under acidic conditions.
Sample : 20μL
5ng/mL each (in H2O)
Column : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm) Eluent : 250mM HCOOH aq./CH3CN=70/30 Flow rate : 0.3mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40°C