Five kinds of monoamine neurotransmitters were analyzed using HILICpak VC-50 2D, a polymer based column for HILIC mode. Histamine is more basic than other monoamines and causing a stronger ion exchange interactions between histamine and gel material resulting histamine to elute later.
0.1μM each (in H2O), 20μL
Column : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm) Eluent : (A) 200mM HCOOH aq. (B) CH3CN Linear gradient (High pressure) ; B% = 60% (0 to 5min) 60% to 10% (5 to 6min) 10% (6 to 20min) Flow rate : 0.3mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40℃