Acarbose, miglitol and voglibose are amino sugars each possessing amino groups, and α-glucosidase inhibitors which have been used as oral anti-diabetes drugs. Metformin contains multiple amines and falls under the biguanide drug family; which is known as oral anti-diabetes drug. In this application, those drugs and metformin were simultaneous analyzed using HILICpak VC-50 2D, a polymer-based column for HILIC mode.
5ng/mL each (in H2O), 5μL
Column : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm) Eluent : (A) 200mM HCOOH aq. (B) CH3CN Linear gradient (High pressure) ; B% = 60% (0 to 5min) 60% to 20% (5 to 6min) 20% (6 to 20min) Flow rate : 0.3mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40°C