LC/MS/MS Analysis of Oral Anti-diabetes Drugs (VC-50 2D)

Acarbose, miglitol and voglibose are amino sugars each possessing amino groups, and α-glucosidase inhibitors which have been used as oral anti-diabetes drugs. Metformin contains multiple amines and falls under the biguanide drug family; which is known as oral anti-diabetes drug. In this application, those drugs and metformin were simultaneous analyzed using HILICpak VC-50 2D, a polymer-based column for HILIC mode.

Sample :
5ng/mL each (in H₂O), 5μL
Acarbose
Miglitol
Voglibose
Metformin

Column       : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm)
Eluent       : (A) 200mM HCOOH aq.
               (B) CH3CN
               Linear gradient (High pressure) ;
               B% = 60% (0 to 5min)
                    60% to 20% (5 to 6min)
                    20% (6 to 20min)
Flow rate    : 0.3mL/min
Detector     : ESI-MS/MS (MRM Positive)
Column temp. : 40°C