Various phosphorylated saccharides are synthesized as intermediates in the glycolytic pathways, like gluconeogenesis or glycometabolism. Four kinds of phosphorylated saccharides were analyzed by HILICpak VT-50 2D under the conditions of LC/MS. This analytical condition can be also used for other detectors like RI, ELSD, and corona CAD.
Sample: 1µM each, 5μL
1. Glucose-6-phosphate (G6P)
2. Fructose-6-phosphate (F6P)
3. Glucose-1-phosphate (G1P)
4. Fructose-1-phosphate (F1P)
Column : Shodex HILICpak VT-50 2D (2.0mmI.D. x 150mm) Eluent : 25mM HCOONH4 aq./CH3CN=80/20 Flow rate : 0.3mL/min Detector : ESI-MS (SIM Negative : m/z 259) Column temp. : 60°C
