HILICpak VG-50 2D, a polymer based amino column, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, and is effective for high sensitive detection.
Sample : 50ng/mL each
(in H2O/CH3CN=1/9), 5μL
Column : Shodex HILICpak VG-50 2D (2.0mmI.D. x 150mm) Eluent : (A); 0.1% NH3 aq. (B); CH3CN Linear gradient ; (B%) 95% (0 to 5min), 95% to 80% (5 to 15min), 80% (15 to 20min) Flow rate : 0.3mL/min Detector : ESI-MS/MS (MRM Negative) Column temp. : 60°C