LC/MS/MS Analysis of Saccharides (VG-50 2D)

HILICpak VG-50 2D, a polymer based amino column, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, and is effective for high sensitive detection.

Sample : 50ng/mL each
(in H₂O/CH₃CN=1/9), 5μL
meso-Erythritol
Arabinose
Xylose
Fructose
Mannose
Glucose
Sucrose
Lactose
Maltose

Column       : Shodex HILICpak VG-50 2D (2.0mmI.D. x 150mm)
Eluent       : (A); 0.1% NH3 aq. (B); CH3CN
               Linear gradient ; (B%) 95% (0 to 5min), 95% to 80% (5 to 15min), 80% (15 to 20min)
Flow rate    : 0.3mL/min
Detector     : ESI-MS/MS (MRM Negative)
Column temp. : 60°C