Salacia extract has been suggested to suppresses the absorption of sugar and inhibits the increase of blood sugar level. In this application, neokotalanol and neosalacinol in aqueous extract from commercially available salacia extract-containing supplements which are analyzed by LC/MS/MS method using HILICpak VC-50 2D, a polymer-based HILIC column.
1) Grind a piece of commercially available salacia extract-containing supplement in a mortar and pestle to a fine powder.
2) Add 1 mL of ultrapure water to 0.1 g of powder and extract the substances using an ultrasonic vibrations for 10 minutes.
3) After centrifugation, dilute the supernatant by 10,000 fold with ultra-pure water.
Sample : 5μL
Column : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm) Eluent : 50mM HCOOH aq./CH3CN=70/30 Flow rate : 0.2mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40°C