Lipoxidase, an oxidoreductase was analyzed using IEC
SP-825 ( a column for strong anion exchange chromatography ). It is a glycoprotein of MW 80,000. Lipoxidase from soybean consists of three fractions. Enzyme activity was found at two peaks around 25 minutes.
Sample: 0.5% Lipoxidase, 150 μL
Column : Shodex IEC SP-825 (8.0mmID*75mm)
Eluent : (A); 20mM Citric acid-NaOH buffer(pH4.3)
(B); (A) + 0.5M Na2SO4
Linear gradient: 0min to 60min, (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : 25°C
